Tilapia Skin Peptides Inhibit Apoptosis, Inflammation, and Oxidative Stress to Improve Dry Eye Disease In Vitro and In Vivo
نویسندگان
چکیده
Dry eye disease (DED) is a common chronic ophthalmic disorder, and there no effective treatment to cure it. It urgent find new antidry compounds. Here, the effects underlying mechanisms of tilapia skin peptides (TSP) on DED were investigated. In vitro, human corneal epithelial cells (HCECs) cultured in hypertonic medium containing TSP for 12 h. The MTT assay, calcein-AM/PI staining, reactive oxygen (ROS) analysis, western blot performed at end experiments. vivo model DED, mice eyes dropped with 0.3% benzalkonium chloride (BAC) 0.1% 14 days. Mice treated BAC twice daily 10-hour time interval between treatments. After treatment, phenolic red cotton experiment, tear ferning test, histology assay carried out. significantly restored cell viability HCECs challenged by NaCl. vivo, secretion function fern-like crystal levels considerably improved group after TSP. Mechanically, impend development regulating Bax/Bcl-2 signal pathway, inhibiting iNOS COX-2 protein expression, moderating ROS/Nrf2/HO-1 axis, apoptosis regions, increasing number conjunctival goblet cells, arresting thinning. conclusion, ameliorated DED-like disorder BAC-induced damage NaCl-induced HCECs. suppressing apoptosis, inflammation, oxidative stress. Consequently, this study reveals beneficial activity DED.
منابع مشابه
Naringin attenuates diabetic retinopathy by inhibiting inflammation, oxidative stress and NF-κB activation in vivo and in vitro
Objective(s): Naringin, an essential flavonoid, inhibits inflammatory response and oxidative stress in diabetes. However, whether naringin has beneficial effects on diabetic retinopathy (DR) remains unknown. Materials and Methods: Streptozotocin (STZ, 65 mg/kg) was intraperitoneally injected into male rats (8 weeks old weighting 200-250 g) to establish diabetic model, then naringin (20, 40 or 8...
متن کاملDry eye disease and inflammation
Dry eye disease is a devastating and multifactorial disorder of the tear-film layer and ocular surface. Multiple symptoms include multiple eye irritation, and visual disturbances, and signs have tear-film instability, along with the potential for injuring the ocular surface. Accumulating evidence indicates that dry eye disease is accompanied by hyperosmolarity of the tear film and inflammation ...
متن کاملCerulein pancreatitis: oxidative stress, inflammation, and apoptosis.
Cerulein pancreatitis is similar to human edematous pancreatitis, manifesting with dysregulation of digestive enzyme production and cytoplasmic vacuolization, the death of acinar cells, edema formation, and infiltration of inflammatory cells into the pancreas. Reactive oxygen species are involved in nuclear factor-kappaB activation, cytokine expression, apoptosis and pathogenesis of pancreatiti...
متن کاملmanipulation in dubbing and subtitling
پژوهش حاضر در چارچوب مکتب دستکاری قرار گرفت و با استفاده از تقسیم بندی دوکات (2007) از شیوه های دستکاری، به دنبال یافتن پاسخ برای پرسش های زیر بود: 1-رایج ترین شیوه دستکاری در دوبله فیلم ها کدام است؟ 2-رایج ترین شیوه دستکاری در زیرنویس فیلم ها کدام است؟ 3-دستکاری در دوبله فیلم ها رایج تر است یا در زیرنویس آن ها؟ این پژوهش از نوع تحقیقات توصیفی- مقایسه ای و پیکره ای می باشد. پیکره تحقیق شا...
ذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Food Biochemistry
سال: 2023
ISSN: ['0145-8884', '1745-4514']
DOI: https://doi.org/10.1155/2023/6761792